Human STAT5B(Signal Transducer And Activator Of Transcription 5B) ELISA Kit

Item number: ELK5146
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Human STAT5B(Signal Transducer And Activator Of Transcription 5B) ELISA Kit

Human STAT5B(Signal Transducer And Activator Of Transcription 5B) ELISA Kit

$265.00

Human STAT5B(Signal Transducer And Activator Of Transcription 5B) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STAT5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STAT5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STAT5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STAT5B in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P51692
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;Hormone metabolism;
Assay length
3.5h
Sample type
Tissue homogenates, cell lysates and other biological fluids
Detection range
0.32-20 ng/mL
Standard
20 ng/mL
Sensitivity
0.113 ng/mL
Assay type
Sandwich
Reactivity
Human

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