Human UCHL1(Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

Item number: ELK7504
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Human UCHL1(Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

Human UCHL1(Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

$265.00

Human UCHL1(Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UCHL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UCHL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UCHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UCHL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P09936
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Enzyme & Kinase;
Assay length
3.5h
Sample type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
0.79-50 ng/mL
Standard
50 ng/mL
Sensitivity
0.28 ng/mL
Assay type
Sandwich
Reactivity
Human

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