Laminin-111 protein plays an important role in embryonic development. iMatrix-111 is unique in that no pre-coating steps are necessary. This kit comes with 2 vials for a total of 0.35 mg of protein. Concentration: 0.5 mg/mL. Laminin 111 supports the survival, proliferation, and differentiation of many different cell types in vitro. However, its distribution after birth is restricted to only a few tissues, such as the brain and kidney. During development, Riechert’s membrane supporting the extraembryonic trophoblasts express laminin 111. Reichert’s membrane is essential for epiblast differentiation, which is essential for the formation of the three embryonic germ layers by pluripotent cells in the inner cell mass. In the adult, laminin α1 chain expression is relatively restricted and is only present in some epithelial basement membranes, and primarily found in tissues such as the eye, liver, and kidney, whereas hardly expressed at all in endothelial, adipose, nervous, or muscle tissues.