Lifeink 200 is a 35 mg/ml type I collagen bioink for extrusion based FRESH 3D bioprinting. Lifeink 200 comes as a pH neutral, isotonic bioink and is ready for cell addition and bioprinting. For printing with Lifeink 200, we highly recommend using the FRESH printing technique and printing at 2-8°C. 3D gels allow for the study of the effects of the mechanical properties of the ECM, such as density and rigidity, on cell development, migration, and morphology. Unlike 2D systems, 3D environments allow cell extensions to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways. Gel stiffness or rigidity also affects cell migration differently in 3D versus 2D environments. A bioink that resembles life should include the following traits: High Print Resolution, shear-thinning, strong mechanical strength, superior cytocompatibility, allow for cellular remodeling, and is biomimetic. Lifeink 200 fulfills those requirements.