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Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit
Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit

Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit

Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8374

$320.00
$320.00
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Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
2.35-150 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Mouse
Research area
Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Sample type
Serum, plasma and other biological fluids.
Sensitivity
1.1 ng/mL
Standard
150 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit

Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit

$320.00
$320.00