Mouse ICAM1(Intercellular Adhesion Molecule 1) ELISA Kit

Item number: ELK1034
Availability: In Stock
Product Type: Precoated
$376.00
$376.00
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Size: 96T
Subtotal: $376.00
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Mouse ICAM1(Intercellular Adhesion Molecule 1) ELISA Kit

Mouse ICAM1(Intercellular Adhesion Molecule 1) ELISA Kit

$376.00

Mouse ICAM1(Intercellular Adhesion Molecule 1) ELISA Kit

$376.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ICAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ICAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ICAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P13597
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
CD & Adhesion molecule;Tumor immunity;Infection immunity;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
0.32-20 ng/mL
Standard
20 ng/mL
Sensitivity
0.122 ng/mL
Assay type
Sandwich
Reactivity
Mouse

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