Mouse OGDH(Oxoglutarate Dehydrogenase) ELISA Kit

Item number: ELK9992
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Mouse OGDH(Oxoglutarate Dehydrogenase) ELISA Kit

Mouse OGDH(Oxoglutarate Dehydrogenase) ELISA Kit

$458.00

Mouse OGDH(Oxoglutarate Dehydrogenase) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OGDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OGDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OGDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OGDH in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Enzyme & Kinase;
Assay length
3.5h
Sample type
Tissue homogenates, cell lysates and other biological fluids
Detection range
3.13-200 ng/mL
Standard
200 ng/mL
Sensitivity
1.15 ng/mL
Assay type
Sandwich
Reactivity
Mouse

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