Mouse OPN(Osteopontin) ELISA Kit

Item number: ELK10183
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Mouse OPN(Osteopontin) ELISA Kit

Mouse OPN(Osteopontin) ELISA Kit

$265.00

Mouse OPN(Osteopontin) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OPN in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P10923
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Tumor immunity;Bone metabolism;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
3.13-200 ng/mL
Standard
200 ng/mL
Sensitivity
1.33 ng/mL
Assay type
Sandwich
Reactivity
Mouse

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