Mouse PF4(Platelet Factor 4) ELISA Kit

Item number: ELK10224
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Mouse PF4(Platelet Factor 4) ELISA Kit

Mouse PF4(Platelet Factor 4) ELISA Kit

$265.00

Mouse PF4(Platelet Factor 4) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q9Z126
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Cytokine;Hematology;
Assay length
3.5h
Sample type
serum, platelet-poor plasma and cell culture supernates
Detection range
15.63-1000 ng/mL
Standard
1000 ng/mL
Sensitivity
6.1 ng/mL
Assay type
Sandwich
Reactivity
Mouse

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