Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit

Item number: ELK1293
Availability: In Stock
Product Type: Precoated
$376.00
$376.00
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Size: 96T
Subtotal: $376.00
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Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit

Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit

$376.00

Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit

$376.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q00731
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Cytokine;Apoptosis;Tumor immunity;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
15.63-1000 pg/mL
Standard
1000 pg/mL
Sensitivity
5.7 pg/mL
Assay type
Sandwich
Reactivity
Mouse

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