Pig BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

Item number: ELK8978
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Pig BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

Pig BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

$320.00

Pig BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig BALP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig BALP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig BALP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig BALP in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Enzyme & Kinase;Metabolic pathway;Hepatology;Bone metabolism;
Assay length
3.5h
Sample type
serum, plasma and other biological fluids
Detection range
1.25-80 ng/mL
Standard
80 ng/mL
Sensitivity
0.5 ng/mL
Assay type
Sandwich
Reactivity
Pig

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