The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Q07717

around 3 working days

L 16cm x W 15cm x H 9cm

Infection immunity;Kidney biomarker;

3.5h

Serum, plasma, urine and other biological fluids

3.13-200 ng/mL

200 ng/mL

1.24 ng/mL

Sandwich

Pig