The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Q45KW7

around 3 working days

L 16cm x W 15cm x H 9cm

Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;

3.5h

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

15.63-1000 pg/mL

1000 pg/mL

6.6 pg/mL

Sandwich

Pig