Rabbit TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TIMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TIMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TIMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

P20614

around 3 working days

L 16cm x W 15cm x H 9cm

Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;Hormone metabolism;

3.5h

Serum, plasma and other biological fluids.

3.13-200 ng/mL

200 ng/mL

1.01 ng/mL

Sandwich

Rabbit