Rat 5HTR7(5-Hydroxytryptamine Receptor 7) ELISA Kit

Item number: ELK9463
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Rat 5HTR7(5-Hydroxytryptamine Receptor 7) ELISA Kit

Rat 5HTR7(5-Hydroxytryptamine Receptor 7) ELISA Kit

$320.00

Rat 5HTR7(5-Hydroxytryptamine Receptor 7) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat 5HTR7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 5HTR7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat 5HTR7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 5HTR7 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P32305
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Neuro science;
Assay length
3.5h
Sample type
Tissue homogenates and other biological fluids.
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.058 ng/mL
Assay type
Sandwich
Reactivity
Rat

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