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Rat APEX1(Apurinic/Apyrimidinic Endonuclease 1) ELISA Kit

Item number: ELK8945
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
Please hurry! Only 100 left in stock

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Size: 48T
Subtotal: $320.00
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Rat APEX1(Apurinic/Apyrimidinic Endonuclease 1) ELISA Kit

Rat APEX1(Apurinic/Apyrimidinic Endonuclease 1) ELISA Kit

$320.00

Rat APEX1(Apurinic/Apyrimidinic Endonuclease 1) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APEX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APEX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APEX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APEX1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P43138
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Enzyme & Kinase;
Assay length
3.5h
Sample type
tissue homogenates, cell lysates and other biological fluids
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.058 ng/mL
Assay type
Sandwich
Reactivity
Rat

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