Rat EDN4(Exendin-4) ELISA Kit

Item number: ELK8653
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Rat EDN4(Exendin-4) ELISA Kit

Rat EDN4(Exendin-4) ELISA Kit

$265.00

Rat EDN4(Exendin-4) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EDN4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EDN4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EDN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EDN4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Diabetes
Assay length
3.5h
Sample type
serum, plasma and other biological fluids
Detection range
15.63-1000 pg/mL
Standard
1000 pg/mL
Sensitivity
6 pg/mL
Assay type
Sandwich
Reactivity
Rat

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