Rat Kim1(Kidney Injury Molecule 1) ELISA Kit

Item number: ELK11099
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Rat Kim1(Kidney Injury Molecule 1) ELISA Kit

Rat Kim1(Kidney Injury Molecule 1) ELISA Kit

$320.00

Rat Kim1(Kidney Injury Molecule 1) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Kim1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Kim1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Kim1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Kim1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
O54947
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Tumor immunity;Infection immunity;Kidney biomarker;
Assay length
3.5h
Sample type
tissue homogenates, urine, cell culture supernates and other biological fluids
Detection range
0.32-20 ng/mL
Standard
20 ng/mL
Sensitivity
0.135 ng/mL
Assay type
Sandwich
Reactivity
Rat

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