Rat NDN(Necdin) ELISA Kit

Item number: ELK9284
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Rat NDN(Necdin) ELISA Kit

Rat NDN(Necdin) ELISA Kit

$320.00

Rat NDN(Necdin) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NDN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NDN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NDN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NDN in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Tumor immunity;
Assay length
3.5h
Sample type
Tissue homogenates and other biological fluids.
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.066 ng/mL
Assay type
Sandwich
Reactivity
Rat

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