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Rat PDK1(Pyruvate Dehydrogenase Kinase Isozyme 1) ELISA Kit
Rat PDK1(Pyruvate Dehydrogenase Kinase Isozyme 1) ELISA Kit

Rat PDK1(Pyruvate Dehydrogenase Kinase Isozyme 1) ELISA Kit

Rat PDK1(Pyruvate Dehydrogenase Kinase Isozyme 1) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK7111

$320.00
$320.00
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Documents

Manual
SDS

Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
1.57-100 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
Enzyme & Kinase;
Sample type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.61 ng/mL
Standard
100 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PDK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PDK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PDK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q63065
Rat PDK1(Pyruvate Dehydrogenase Kinase Isozyme 1) ELISA Kit

Rat PDK1(Pyruvate Dehydrogenase Kinase Isozyme 1) ELISA Kit

$320.00
$320.00