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Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit
Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit

Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit

Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8404

$320.00
$320.00
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Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
62.5-4000 pg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
Cytokine;Tumor immunity;Infection immunity;
Sample type
serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
17.2 pg/mL
Standard
4000 pg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.
Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit

Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit

$320.00
$320.00