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Rat SEMA3A(Semaphorin 3A) ELISA Kit
Rat SEMA3A(Semaphorin 3A) ELISA Kit

Rat SEMA3A(Semaphorin 3A) ELISA Kit

Rat SEMA3A(Semaphorin 3A) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK7966

$320.00
$320.00
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Documents

Manual
SDS

Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
15.63-1000 pg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
CD & Adhesion molecule;
Sample type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
6.8 pg/mL
Standard
1000 pg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SEMA3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SEMA3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SEMA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SEMA3A in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q63548
Rat SEMA3A(Semaphorin 3A) ELISA Kit

Rat SEMA3A(Semaphorin 3A) ELISA Kit

$320.00
$320.00