Sheep IL12(Interleukin 12) ELISA Kit

Item number: ELK9130
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Sheep IL12(Interleukin 12) ELISA Kit

Sheep IL12(Interleukin 12) ELISA Kit

$265.00

Sheep IL12(Interleukin 12) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep IL12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep IL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep IL12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Cytokine;Infection immunity;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
31.3-2000 pg/mL
Standard
2000 pg/mL
Sensitivity
12.5 pg/mL
Assay type
Sandwich
Reactivity
Sheep

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