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Sheep SPB(Surfactant Associated Protein B) ELISA Kit
Sheep SPB(Surfactant Associated Protein B) ELISA Kit

Sheep SPB(Surfactant Associated Protein B) ELISA Kit

Sheep SPB(Surfactant Associated Protein B) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8875

$320.00
$320.00
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Manual
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Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
1.57-100 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Sheep
Research area
Tumor immunity;Infection immunity;Pulmonology;
Sample type
serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.56 ng/mL
Standard
100 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep SPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep SPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep SPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep SPB in the samples is then determined by comparing the OD of the samples to the standard curve.
Sheep SPB(Surfactant Associated Protein B) ELISA Kit

Sheep SPB(Surfactant Associated Protein B) ELISA Kit

$320.00
$320.00