Zebrafish GSTs(Glutathione S Transferases) ELISA Kit

Item number: ELK9306
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Zebrafish GSTs(Glutathione S Transferases) ELISA Kit

Zebrafish GSTs(Glutathione S Transferases) ELISA Kit

$320.00

Zebrafish GSTs(Glutathione S Transferases) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish GSTs. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish GSTs. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish GSTs, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish GSTs in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q50LC6
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Enzyme & Kinase;Metabolic pathway;Tumor immunity;Hepatology;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.056 ng/mL
Assay type
Sandwich
Reactivity
Zebrafish

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