12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

Item number: ELK8156
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

$265.00

12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

$265.00
Size: 48T
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Assay length
2h
Sample type
serum, plasma and other biological fluids
Detection range
15.63-1000 ng/mL
Standard
1000 ng/mL
Sensitivity
5.13 ng/mL
Assay type
Competitive Inhibition
Reactivity
General

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