12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit
12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8156

$265.00 USD
Shipping calculated at checkout.

Available in stock (100)

  • Customs clearance fees may apply
  • Worldwide shipping available.
For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
2h
Assay type
Competitive Inhibition
Detection range
15.63-1000 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
General
Research area
Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Sample type
serum, plasma and other biological fluids
Sensitivity
5.13 ng/mL
Standard
1000 ng/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

$265.00 USD