Western Blot, Positive WB detected in: HepG2 whole cell lysate (treated by 30mM sodium butyrate for 4h), All lanes: HIST1H3A antibody at 2.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15412 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15412) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
2-hydroxyisobutyryl-HIST1H3A (K23) Polyclonal Antibody - image 4
Western Blot, Positive WB detected in: HepG2 whole cell lysate (treated by 30mM sodium butyrate for 4h), All lanes: HIST1H3A antibody at 2.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15412 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15412) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
2-hydroxyisobutyryl-HIST1H3A (K23) Polyclonal Antibody - image 4

2-hydroxyisobutyryl-HIST1H3A (K23) Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC15412-100UL

$544.38 USD
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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P68431
Applications
ELISA, WB, IF, ChIP
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The 2-hydroxyisobutyryl-HIST1H3A (K23) Polyclonal Antibody is applicable for the following applications: ELISA, WB, IF, ChIP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
Peptide sequence around site of 2-hydroxyisobutyryl-Lys (23) derived from Human Histone H3.1
Isotype
IgG
Purification
Antigen affinity purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics, Nuclear Signaling
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
HIST1H3A
Target synonyms
H3 histone family member E pseuDog (Canis familiaris; Canine)ene; H3 histone family; member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1; H3a; Histone cluster 1; H3a; Histone H3 3 pseuDog (Canis familiaris; Canine)ene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Western Blot, Positive WB detected in: HepG2 whole cell lysate (treated by 30mM sodium butyrate for 4h), All lanes: HIST1H3A antibody at 2.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa

2-hydroxyisobutyryl-HIST1H3A (K23) Polyclonal Antibody

$544.38 USD