25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK9387

$265.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
2h
Assay type
Competitive Inhibition
Detection range
3.13-200 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
General
Research area
Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Sample type
serum, plasma and other biological fluids
Sensitivity
0.9 ng/mL
Standard
200 ng/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

$265.00 USD