4-HNE(4-Hydroxynonenal) ELISA Kit
4-HNE(4-Hydroxynonenal) ELISA Kit

4-HNE(4-Hydroxynonenal) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK9581

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$320.00 USD
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For research use only — not intended for clinical or diagnostic purposes.
4-HNE(4-Hydroxynonenal) ELISA Kit

4-HNE(4-Hydroxynonenal) ELISA Kit

$320.00 USD

THE SCIENCE, IN DETAIL

Know your product.

Specifications
Assay length
2h
Assay type
Competitive Inhibition
Detection range
0.63-40 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
General
Research area
Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Sample type
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.38 ng/mL
Standard
40 ng/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical documents

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