8-iso-PGF2α(8-isoprostane) ELISA Kit

Item number: ELK0151
Availability: In Stock
Product Type: Precoated
$458.00
$0.00
$458.00
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Size: 96T
Subtotal: $458.00
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8-iso-PGF2α(8-isoprostane) ELISA Kit

8-iso-PGF2α(8-isoprostane) ELISA Kit

$0.00 $458.00

8-iso-PGF2α(8-isoprostane) ELISA Kit

$0.00 $458.00
Size: 96T
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Assay length
2.5h
Sample type
serum, plasma and other biological fluids
Detection range
15.63-1000 pg/mL
Standard
1000 pg/mL
Sensitivity
4.47 pg/mL
Assay type
Competitive Inhibition
Reactivity
General

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