Western Blot; Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate; All lanes: ACTN1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 104, 103, 106, 108 kDa; Observed band size: 104 kDa;
Immunofluorescence staining of Hela cells with CAC11647 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Actn1 Polyclonal Antibody - image 3
Western Blot; Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate; All lanes: ACTN1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 104, 103, 106, 108 kDa; Observed band size: 104 kDa;
Immunofluorescence staining of Hela cells with CAC11647 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Actn1 Polyclonal Antibody - image 3

Actn1 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC11647-100UL

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P12814
Applications
ELISA, WB, IF
Buffer
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Actn1 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
Recombinant Human Alpha-Actinin-1 Protein (1-204Aa)
Isotype
IgG
Purification
>95%, Protein G Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Signal Transduction
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
ACTN1
Target synonyms
actinin 1 smooth muscle; Actinin alpha 1; actinin; alpha 1; ACTN 1; Actn1; ACTN1_HUMAN; Alpha Actinin 1; Alpha actinin cytoskeletal isoform; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; BDPLT15; F actin cross linking protein; F-actin cross-linking protein; FLJ40884; FLJ54432; Non muscle alpha actinin 1; Non-muscle alpha-actinin-1
Western Blot; Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate; All lanes: ACTN1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 104, 103, 106, 108 kDa; Observed band size: 104 kDa;

Actn1 Polyclonal Antibody

$510.25 USD