ADP(Adenosine Diphosphate) ELISA Kit
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$265.00 USD
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For research use only — not intended for clinical or diagnostic purposes.
ADP(Adenosine Diphosphate) ELISA Kit
Regular price
$265.00 USD
Sale price
Regular price
Unit price
/per
THE SCIENCE, IN DETAIL
Know your product.
Specifications+
Assay length
2h
Assay type
Competitive Inhibition
Detection range
23.44-1500 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
General
Research area
Signal transduction;Metabolic pathway;Developmental science;
Sample type
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
7.19 ng/mL
Standard
1500 ng/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical documents+
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