Immunohistochemistry of paraffin-embedded human kidney tissue using CAC07145 at dilution of 1:100
IHC image diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot; , All lanes: Protein argonaute-2 antibody at 2µg/ml; , Lane 1: A549 whole cell lysate; , Lane 2: Jurkats whole cell lysate; , Lane 3: MCF-7 whole cell lysate; , Lane 4: HepG2 whole cell lysate; , Lane 5: Raw264.7 whole cell lysate; , Lane 6: K562 whole cell lysate; , Lane 7: Mouse liver tissue; , Lane 8: Mouse kidney tissue; , Secondary; , Goat polyclonal to rabbit IgG at 1/10000 dilution; , Predicted band size: 98, 94 kDa; , Observed band size: 94 kDa
Ago2 Polyclonal Antibody - image 5
Immunohistochemistry of paraffin-embedded human kidney tissue using CAC07145 at dilution of 1:100
IHC image diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot; , All lanes: Protein argonaute-2 antibody at 2µg/ml; , Lane 1: A549 whole cell lysate; , Lane 2: Jurkats whole cell lysate; , Lane 3: MCF-7 whole cell lysate; , Lane 4: HepG2 whole cell lysate; , Lane 5: Raw264.7 whole cell lysate; , Lane 6: K562 whole cell lysate; , Lane 7: Mouse liver tissue; , Lane 8: Mouse kidney tissue; , Secondary; , Goat polyclonal to rabbit IgG at 1/10000 dilution; , Predicted band size: 98, 94 kDa; , Observed band size: 94 kDa
Ago2 Polyclonal Antibody - image 5

Ago2 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC07145-100UG

$510.25 USD
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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q9UKV8
Applications
ELISA, WB, IHC, ChIP
Buffer
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Ago2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, ChIP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Protein Argonaute-2 Protein (517-818Aa)
Isotype
IgG
Purification
>95%, Protein G Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
AGO2
Target synonyms
Ago 2; AGO2_HUMAN; Argonaute 2; argonaute 2; RISC catalytic component; Argonaute RISC catalytic component 2; Argonaute2; CTA-204B4.6; dAgo2; eIF 2C 2; eIF-2C 2; eIF2C 2; Eif2c2; Eukaryotic translation initiation factor 2C 2; Eukaryotic translation initiation factor 2C subunit 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10; Slicer protein
Immunohistochemistry of paraffin-embedded human kidney tissue using CAC07145 at dilution of 1:100

Ago2 Polyclonal Antibody

$510.25 USD