Western Blot, Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, Rat heart tissue, All lanes: ANO2 antibody at 3.9ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 114 kDa, Observed band size: 114 kDa
IHC image of CAC15336 diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC15336 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ANO2 Polyclonal Antibody - image 4
Western Blot, Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, Rat heart tissue, All lanes: ANO2 antibody at 3.9ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 114 kDa, Observed band size: 114 kDa
IHC image of CAC15336 diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC15336 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ANO2 Polyclonal Antibody - image 4

ANO2 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC15336-100UG

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q9NQ90
Applications
ELISA, WB, IHC, IF
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The ANO2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Anoctamin-2 protein (1-83AA)
Isotype
IgG
Purification
>95%, Protein G purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Neuroscience
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Rat (Rattus norvegicus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
ANO2
Target synonyms
Ano2; ANO2_HUMAN; Anoctamin 2; Anoctamin-2; C12orf3; Chromosome 12 open reading frame 3; DKFZp434P102; Transmembrane protein 16B (eight membrane-spanning domains); Transmembrane protein 16B
Western Blot, Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, Rat heart tissue, All lanes: ANO2 antibody at 3.9ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 114 kDa, Observed band size: 114 kDa

ANO2 Polyclonal Antibody

$510.25 USD