Western Blot; Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, Rat lung tissue, Rat brain tissue, Mouse lung tissue, Mouse brain tissue; All lanes: BCAP31 antibody at 4.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 28, 35 kDa; Observed band size: 28 kDa
IHC image diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:1200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC09027 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Bcap31 Polyclonal Antibody - image 5
Western Blot; Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, Rat lung tissue, Rat brain tissue, Mouse lung tissue, Mouse brain tissue; All lanes: BCAP31 antibody at 4.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 28, 35 kDa; Observed band size: 28 kDa
IHC image diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:1200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC09027 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Bcap31 Polyclonal Antibody - image 5

Bcap31 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC09027-100UG

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P51572
Applications
ELISA, WB, IHC, IF, IP
Buffer
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Bcap31 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human B-Cell Receptor-Associated Protein 31 Protein (2-243Aa)
Isotype
IgG
Purification
>95%, Protein G Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cell Biology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
BCAP31
Target synonyms
6C6 AG; 6C6 AG tumor associated antigen; 6C6-AG tumor-associated antigen; 6C6AG; 6C6AG tumor associated antigen; Accessory protein BAP 31; Accessory protein BAP31; B cell receptor associated protein 31; B-cell receptor-associated protein 31; BA31; BAP 31; Bap31; BAP31_HUMAN; BCAP 31; BCAP31; BCR associated protein Bap 31; BCR associated protein Bap31; BCR-associated protein 31; CDM; CDM protein; DXS1357E; MS950; p28; p28 Bap31; Protein CDM; RP23-329M9.5
Western Blot; Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, Rat lung tissue, Rat brain tissue, Mouse lung tissue, Mouse brain tissue; All lanes: BCAP31 antibody at 4.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 28, 35 kDa; Observed band size: 28 kDa

Bcap31 Polyclonal Antibody

$510.25 USD