Biotinylated Human Frizzled-8 (C-6His-Avi)
Vendor: ELK Biotechnology
SKU: EPT227
Regular price
$1,439.00 USD
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Regular price
Unit price
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For research use only — not intended for clinical or diagnostic purposes.
Product Overview
Product Overview
Accession
Q9H461
Apparent molecular weight
25-35 KDa, reducing conditions
Delivery condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Description
Biotinylated Recombinant Human Frizzled-8 is produced by our Mammalian expression system and the target gene encoding Ala28-Pro172 is expressed with a 6His, Avi tag at the C-terminus.
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Molecular weight
19.1 KDa
Other names
frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Redissolve
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
Background
Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.
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Product Citations
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More Info
More Info
Biotinylated Human Frizzled-8 (C-6His-Avi)
Regular price
$1,439.00 USD
Sale price
Regular price
Unit price
/per

