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Cattle IgM(Immunoglobulin M) ELISA Kit
Cattle IgM(Immunoglobulin M) ELISA Kit

Cattle IgM(Immunoglobulin M) ELISA Kit

Cattle IgM(Immunoglobulin M) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK4256

$588.00
$588.00
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Manual
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Available in stock (100)

Size: 96T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
1.57-100 µg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Cattle
Research area
Infection immunity;Immune molecule;Hematology;
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.71 µg/mL
Standard
100 µg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
Cattle IgM(Immunoglobulin M) ELISA Kit

Cattle IgM(Immunoglobulin M) ELISA Kit

$588.00
$588.00