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Cattle MPO(Myeloperoxidase) ELISA Kit
Cattle MPO(Myeloperoxidase) ELISA Kit

Cattle MPO(Myeloperoxidase) ELISA Kit

Cattle MPO(Myeloperoxidase) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK10716

$588.00
$588.00
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Manual
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Available in stock (100)

Size: 96T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
15.63-1000 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Cattle
Research area
Enzyme & Kinase;Infection immunity;Cardiovascular biology;Hepatology;
Sample type
Serum, plasma and other biological fluids.
Sensitivity
3.54 ng/mL
Standard
1000 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle MPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle MPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle MPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle MPO in the samples is then determined by comparing the OD of the samples to the standard curve.
Cattle MPO(Myeloperoxidase) ELISA Kit

Cattle MPO(Myeloperoxidase) ELISA Kit

$588.00
$588.00