Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CAC15482 at 1:37.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot, Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, All lanes: HIST1H2BC antibody at 1.8ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunoprecipitating HIST1H2BC in K562 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15482 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15482 (3ug) + K562 whole cell lysate (500ug), Lane 3: K562 whole cell lysate (20ug)
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15482) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Crotonyl-HIST1H2BC (K23) Polyclonal Antibody - image 5
Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CAC15482 at 1:37.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot, Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, All lanes: HIST1H2BC antibody at 1.8ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunoprecipitating HIST1H2BC in K562 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15482 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15482 (3ug) + K562 whole cell lysate (500ug), Lane 3: K562 whole cell lysate (20ug)
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15482) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Crotonyl-HIST1H2BC (K23) Polyclonal Antibody - image 5

Crotonyl-HIST1H2BC (K23) Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC15482-100UL

$544.38 USD
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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P62807
Applications
ELISA, WB, IF, IP, ChIP
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Crotonyl-HIST1H2BC (K23) Polyclonal Antibody is applicable for the following applications: ELISA, WB, IF, IP, ChIP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
Peptide sequence around site of Crotonyl-Lys (23) derived from Human Histone H2B type 1-C/E/F/G/I
Isotype
IgG
Purification
Antigen affinity purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics, Nuclear Signaling
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
HIST1H2BC
Target synonyms
H2BC4; H2BFL; HIST1H2BC; H2BC6; H2BFH; HIST1H2BE; H2BC7; H2BFG; HIST1H2BF; H2BC8; H2BFA; HIST1H2BG; H2BC10; H2BFK; HIST1H2BIHistone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CAC15482 at 1:37.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Crotonyl-HIST1H2BC (K23) Polyclonal Antibody

$544.38 USD