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IP antibody use: 5µg Flag Mouse IgG1 per ml Lysate, WB 1:5000 Lane 1: untransfected 293 cell lysate Lane 2: transfected 293 cell lysate with Flag Tag fusion protein Lane 3: IP (transfected 293 + normal Mouse IgG + Protein G agarose) Lane 4: IP (tr...

Flag Tag Monoclonal Antibody

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