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Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody.

Phospho-AKT1/AKT2/AKT3 (Tyr315/316/312) Antibody

Regular price $360.00 USD
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THE SCIENCE, IN DETAIL

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Specifications
Alternative names
RAC-PK-alpha; Protein kinase B;
Antigen
Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Clone number
N/A
Conjugated
Non-conjugated
Form
liquid
Host species
Rabbit
Isotype
IgG
Purification method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Research area
Neuroscience
Species
Human
Species reactivity
Human, Mouse, Rat
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target name
AKT1/AKT2/AKT3
Tested applications
ELISA, WB, IHC, IF; WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200
Uniprot id
P31749/P31751/Q9Y243
Scientific background
Target: AKT1/AKT2/AKT3. Antigen: Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.. Reactivity: Human, Mouse, Rat. Applications:...

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