Western Blot, Positive WB detected in: Jurkat whole cell lysate, HL60 whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, All lanes: CYP21A2 antibody at 8ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 56, 53 kDa, Observed band size: 56 kDa
IHC image of CAC13022 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 with CAC13022 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CYP21A2 Polyclonal Antibody - image 4
Western Blot, Positive WB detected in: Jurkat whole cell lysate, HL60 whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, All lanes: CYP21A2 antibody at 8ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 56, 53 kDa, Observed band size: 56 kDa
IHC image of CAC13022 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 with CAC13022 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CYP21A2 Polyclonal Antibody - image 4

CYP21A2 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC13022-100UG

$510.25 USD
Shipping calculated at checkout.

Documents

SDS

Available in stock (500)

  • Customs clearance fees may apply
  • Worldwide shipping available.
For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P08686
Applications
ELISA, WB, IHC, IF
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The CYP21A2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Steroid 21-hydroxylase protein (312-418AA)
Isotype
IgG
Purification
>95%, Protein G purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cardiovascular
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
CYP21A2
Target synonyms
CYP21; 21 OHase; 21-OHase; CA21H; CAH1; CP21A_HUMAN; CPS1; CYP21A 2; CYP21A2; CYP21B; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 C21B; Cytochrome P450 XXI; Cytochrome P450; family 21; subfamily A; polypeptide 2; Cytochrome P450; subfamily XXIA (steroid 21 hydroxylase; congenital adrenal hyperplasia); polypeptide 2; Cytochrome P450-C21; Cytochrome P450-C21B; P450 C21; P450 C21B; P450c21B; Steroid 21 hydroxylase; Steroid 21 monooxygenase; Steroid 21-hydroxylase
Western Blot, Positive WB detected in: Jurkat whole cell lysate, HL60 whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, All lanes: CYP21A2 antibody at 8ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 56, 53 kDa, Observed band size: 56 kDa

CYP21A2 Polyclonal Antibody

$510.25 USD