Western Blot; Positive WB detected in: Rat kidney tissue; All lanes: CYP8B1 antibody at 6.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 59 kDa; Observed band size: 59 kDa;
IHC image diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC11309 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Cyp8B1 Polyclonal Antibody - image 5
Western Blot; Positive WB detected in: Rat kidney tissue; All lanes: CYP8B1 antibody at 6.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 59 kDa; Observed band size: 59 kDa;
IHC image diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC11309 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Cyp8B1 Polyclonal Antibody - image 5

Cyp8B1 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC11309-100UG

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q9UNU6
Applications
ELISA, WB, IHC, IF
Buffer
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Cyp8B1 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human 7-Alpha-Hydroxycholest-4-En-3-One 12-Alpha-Hydroxylase Protein (303-440Aa)
Isotype
IgG
Purification
>95%, Protein G Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cardiovascular
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Rat (Rattus norvegicus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
CYP8B1
Target synonyms
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CP8B; CP8B1_HUMAN; CYP12; CYP8B1; CYPVIIIB1; Cytochrome P450 8B1; Cytochrome P450 subfamily VIIIB (sterol 12 alpha hydroxylase) polypeptide 1; Cytochrome P450; family 8; subfamily B; polypeptide 1; Sterol 12-alpha-hydroxylase
Western Blot; Positive WB detected in: Rat kidney tissue; All lanes: CYP8B1 antibody at 6.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 59 kDa; Observed band size: 59 kDa;

Cyp8B1 Polyclonal Antibody

$510.25 USD