Dog CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit
Dog CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

Dog CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK7338

$412.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Dog
Research area
Signal transduction;CD & Adhesion molecule;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;
Sample type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.057 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
O97626
Dog CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

Dog CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

$412.00 USD