EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
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$588.00 USD
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For research use only — not intended for clinical or diagnostic purposes.
Product Overview
Product Overview
Assay length
1.5h
Assay type
Competitive Inhibition
Detection range
78.13-5000 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Sample type
Serum, plasma
Sensitivity
7.7 ng/mL
Standard
5000 ng/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
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Background
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More Info
EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Regular price
$588.00 USD
Sale price
Regular price
Unit price
/per

