FM Minimal Medium

This media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to its amino acid compostion. Aspartic acid is added. Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7. Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins. Besides these components, there is a large group of amino acids present. To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine. Formula mg/l Amino Acids   Arg 700 Asp 300 Cys 200 GluA 500 Gly 900 His 300 Ile 600 Leu 900 Lys 900 Met 300 Phe 500 Pro 800 Thr 500 Trp 200 Val 700 Vitamins   Biotin 0.02 Cyanocobalamin 0.01 Folic Acid 0.2 Lipoic Acid 0.4 Riboflavin 0.5 Thiamine 0.6 Micro Elements   Na2EDTA.2H2O 4.84 ZnSO4 2.3 H3BO3 1.11 MnCl2.4H2O 0.51 CoCl2.6H2O 0.17 CuSO4.5H2O 0.15 (NH4)6Mo7O24.4H2O 0.1 Minerals   NH4Cl 53.5 CaCl2.2H2O 2.94 FeCl3 16.2 MgCl2.6H2O 81.32 KH2PO4 870 Carbon Source   Glucose 10000 Total 19334.87 Suspend 19.3 gram powdered medium in 1 litre distilled water Store dry at room temperature.