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AIM - 2YT Broth Base w/o Trace elements - 1

Hygromycin B Powder

Regular price $766.86 USD
Sale price Regular price

THE SCIENCE, IN DETAIL

Know your product.

Product overview

Empirical Formula (Hill Notation): C20H37N3O13
Molecular Weight = 527.5

Hygromycin B is an aminoglycoside produced by Streptomyces hygroscopicus. The antibiotic interferes with the translation step of polypeptide synthesis of prokaryotes and eukaryotes. The antibiotic inhibits peptide chain elongation by preventing elongation-factor EF-2 dependent translocation. Hygromycin B is used as a selective agent in yeasts, bacterial- and plant cells. The antibiotic is inactivated by means of incorporation of the APH 4 gene. 

Specifications

 
Appearance White Crystalline Powder
Identification (FTIR) Positive
Solubility (100 mg/L) Clear and colourless solution
Purity (HPLC) >92%
Potency >1000 µg/mg
Heavy metals <20 ppm
Specific rotation 22.1
Endotoxin <10 EU/mg

Store at 2 - 8°C 

CAS 31282-04-9

Specifications
Description
Suspend 45.85 gram powdered medium in 1 litre distilled water
Scientific background
Formula g/l Tryptone 16 Yeast extract 10 (NH4)2SO4 3.3 KH2PO4 6.8 Na2HPO4 7.1 Glucose 0.5 α-Lactose 2.0 MgSO4 0.15 Suspend 45.85 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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