MRS Broth
MRS Medium, named for its inventors De Man, Rogosa and Sharpe is a selective culture medium designed to favour the luxuriant growth of Lactobacilli in bacteriological cell cultures. Although MRS medium is...
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THE SCIENCE, IN DETAIL
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Product overview
MRS Medium, named for its inventors De Man, Rogosa and Sharpe is a selective culture medium designed to favour the luxuriant growth of Lactobacilli in bacteriological cell cultures.
Although MRS medium is selective for lactobacilli but some growth of leuconostocs and pediococci may occur. One of the constituents of MRS Broth is sodium acetate, which suppresses the growth of many competing bacteria.
Yeast extract and meat extract present in MRS Broth provide sources of carbon, nitrogen, and vitamins for general bacterial growth. The yeast extract also contains vitamins and amino acids specifically required by Lactobacilli.
Polysorbate 80 is a surfactant which assists in nutrient uptake by Lactobacilli. Magnesium sulphate and manganese sulphate provide cations used in metabolism.
Formual |
g/l |
| Peptone | 20 |
| Yeast Extract | 5 |
|
Tri-ammonium Citrate
|
2 |
| Magnesium Sulphate | 0.2 |
| Glucose | 20 |
| Tween 80 | 1 |
| Sodium Acetate | 5 |
| Dipotassium Hydrogen Phosphate | 2 |
| Manganese sulphate | 0.05 |
Suspend 55.3g in 1L of Distilled or De-ionised Water
Store at 8-25°C
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