Neidhardt Basal Salt Mixture, Glucose 20 g/L

Containing all micro and macro salt, buffers and glucose.

A mixture of Neidhardt basal salt mixture in a concentration of 32.9 grams/l with Neidhardt supplement mixture in a concentration of 3.1 grams/l.

Suspend 32.9 grams of Neidhardt basal salt mixture plus 3.1 grams of Neidhardt supplement mixture in one litre of distilled water.

Sterilisation by means of filtration.
After dissolving a slight precipitation might occur. It is not clear what this precipitate might be. Removing it from the medium solution by filtration has showed no negative effect on cell growth