Neidhardt Basal Salt Mixture, Glucose 20 g/L
Containing all micro and macro salt, buffers and glucose. A mixture of Neidhardt basal salt mixture in a concentration of 32.9 grams/l with Neidhardt supplement mixture in a concentration of 3.1 grams/l....
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Product overview
Containing all micro and macro salt, buffers and glucose.
A mixture of Neidhardt basal salt mixture in a concentration of 32.9 grams/l with Neidhardt supplement mixture in a concentration of 3.1 grams/l.
Suspend 32.9 grams of Neidhardt basal salt mixture plus 3.1 grams of Neidhardt supplement mixture in one litre of distilled water.
Sterilisation by means of filtration.
After dissolving a slight precipitation might occur. It is not clear what this precipitate might be. Removing it from the medium solution by filtration has showed no negative effect on cell growth
Composition |
mg/l
|
| MOPS | 8,372.00 |
| NaCl | 2,920.00 |
| Tricine | 716.80 |
| NH4Cl2 | 508.25 |
| K2HPO4 | 229.94 |
| MgSO4 | 78.33 |
| K2SO4 | 48.11 |
| FeSO4.7H2O | 2.78 |
| CaCl2 | 0.056 |
| CuSO4.6H2O | 0.025 |
| H3BO3 | 0.025 |
| ZnSO4.7H2O | 0.029 |
| MnSO4.H2O | 0.014 |
| CoCl2.6H2O | 0.007 |
| NaMoO2.2H2O | 0.001 |
| Glucose | 20,000.00 |
| Total | 32,876.37 |
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