Terrific Broth

Terrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E.coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium.

Terrific Broth medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid.

Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen, vitamins and cofactors for a luxurious growth.

Potassium phosphate is present in Terrific broth buffered to balance the pH and subsequently prevent cell death due to a drop in pH.

Tartoff, K. D., and C. A. Hobbs, Improved media for growing plasmids and cosmid clones, Bethesda Research Laboratories, 9, 12, (1987). Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.

Suspend 36 gram powdered medium in 1 litre distilled water

Add 4ml Glycerol

Store dry at room temperature.